All Lifelines participants of 8 years and older participating in general assessment 2A visited a location of their choice. A trained physician's assistant performed the described measurements, according to the Standard Operating Procedure below.

• NB: For the most part of 1A, the Pulmonary function test and Ruff Figural Fluency Test were performed in alternate weeks so that participants underwent only one of these two tests.
  
• NNB: In case of temporary counter indications or technical/logistic problems, tests could be rescheduled for 1A Visit 2.
  

• Total duration: 70 minutes
• Retrieve the participant from the waiting room
• Check informed Consent and basic personal information
• Take in 1A Questionnaire 1 and check for completeness (also during blood pressure measurement)
• If participant is 65+ years old: perform MMSE
• Check if all instruments are ready for use
• Perform the following measurements in order according to their respective SOP’s:
  1. Anthropometry
  2. Blood pressure
  3. ECG
  4. Pulmonary function test or Ruff Figural Fluency Test
  5. MINI neuropsychiatric interview
  6. Skin autofluorescence
• Possible changes in the order:
  • Blood pressure and ECG may be swapped
  • Pulmonary function test may be done after the MINI

• Total duration: 60 min (plus 30-45 minutes for on-site 1A Youth Questionnaire)
• Retrieve the child and its parent(s) from the (child-friendly) waiting room
• Informed Consent must have been checked prior to the visit
• Check basic personal information
• Take in 1A Birth Questionnaire and 1A General Questionnaire (if not already sent by mail, lost, or forgotten)
• Hand out 1A Behavior Questionnaire and 1A Food Questionnaire
• Check if all instruments are ready for use
• Perform the following measurements in order according to their respective SOP’s:
  1. Anthropometry
  2. Blood pressure
  3. ECG (13+ years old)
  4. Pulmonary function test
• Instruct older children (13+) to fill in the digital 1A Youth Questionnaire on site.

1A Visit 1 was accompanied by 1A Questionnaire 1 which was filled in, on average, within xx days (+/- yy days) prior to visit 1.
1A Visit 1 was followed by 1A Visit 2 within, on average, 40 days.

1A Visit 1 is procedurally equivalent to 2A Visit 1 and 3A Visit 1. The average time between 1A Visit 1 and 2A Visit 1 was 3 years and 11 months.

All Lifelines participants participating in general assessment 2A (“Second Assessment”) visited a location of their choice.
At the location, they underwent the following measurements:

• Anthropometric measures (8+ years old)
• Blood pressure (8+ years old)
• 12-lead electrocardiogram (13+ years old)
• MINI neuropsychiatric interview (18+ years old)
• Jump test (8+ years old)
• CogState (13+ years old)
• Pulmonary function test (8+ years old - performed only in the first 60.000 participants)

2A Visit 1 was accompanied by 2A Questionnaire 1 which was filled in, on average, within xx days (+/- yy days) prior to visit 1.
2A Visit 1 was followed by 2A Visit 2 within, on average, 40 days.

2A Visit 1 is procedurally equivalent to 1A Visit 1 and 3A Visit 1. The average time between 1A Visit 1 and 2A Visit 1 was 3 years and 11 months.