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urine_samples [2023/02/14 10:04] kevinurine_samples [2026/09/11 07:37] (current) rick
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 ===== Current Study Protocol ===== ===== Current Study Protocol =====
  
-Please note that the text below describes the procedures as they are employed during the ongoing [[3A|third]] assessment. The [[1A|baseline]]- and [[2A|second]] assessments differ in various ways, and Lifelines recommends consulting their respective wiki pages to learn more about the procedures employed at those times.+Please note that the text below describes the procedures as they are employed during the ongoing [[4A|third]] assessment. The [[1A|baseline]]-[[2A|second]]- and [[3A|third]] assessments differ in various ways, and Lifelines recommends consulting their respective wiki pages to learn more about the procedures employed at those times.
  
 ===== Sample Collection ===== ===== Sample Collection =====
  
-Adult participants were instructed to collect their urine over a full 24-hour period (08:00-08:00) as these samples are considered the "gold standard" to determine conditions such as albuminuria (Gansevoort RT et al. 2006(([[https://pubmed.ncbi.nlm.nih.gov/16936069/|Evaluation of measures of urinary albumin excretion]]))) and assessing kidney function.\\+Adult participants were instructed to collect their urine over a full 24-hour period (22:00-22:00) as these samples are considered the "gold standard" to determine conditions such as albuminuria (Gansevoort RT et al. 2006(([[https://pubmed.ncbi.nlm.nih.gov/16936069/|Evaluation of measures of urinary albumin excretion]]))) and assessing kidney function.\\
 Underage participants (ages 8–17) were instructed to collect their urine over a 12-hour period (20:00-08:00) because of the inconvenience posed by the 3-liter containers.\\ Underage participants (ages 8–17) were instructed to collect their urine over a 12-hour period (20:00-08:00) because of the inconvenience posed by the 3-liter containers.\\
 Participants were instructed to store their containers at room temperature during the sample collection procedure. Participants were instructed to store their containers at room temperature during the sample collection procedure.
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 ===== Sample Processing ===== ===== Sample Processing =====
  
-Urine samples are divided into 900 μl fractions by the [[https://synchronlab.com/product/xperimate-modular-robotic-pipetting-platform/|Synchron Xperimate robot]], a modular robotic pipetting platform. These aliquots can be identified by a unique 2D barcode printed on the bottom of the tubes, and are held within a 1D barcoded rack ([[https://www.lvl-technologies.com/en/sample-storage/safe-2d-tubes-engl/safe-96-it-2D|LVL technologies Safe® 96 IT]] or equivalent). The exact location of each individual sample is logged and continuously tracked in our LIMS.\\+Urine samples are divided into 900 μl aliquots by a Hamilton robotics liquid handler, a modular robotic pipetting platform. These aliquots can be identified by a unique 2D barcode printed on the bottom of the tubes, and are held within a 1D barcoded rack ([[https://www.lvl-technologies.com/en/sample-storage/safe-2d-tubes-engl/safe-96-it-2D|LVL technologies Safe® 96 IT]] or equivalent). The exact location of each individual sample is logged and continuously tracked in our LIMS.\\
  
 ===== Sample Storage ===== ===== Sample Storage =====
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