Differences

This shows you the differences between two versions of the page.

--- urine_samples [2020/09/17 10:43]
trynke
+++ urine_samples [2023/03/22 09:02] (current)
kevin
@@ Line 1: / Line 1: @@
-====== Urine samples ======
+====== Urine Samples ======
-All participants participating in [[1A Visit 2]] and [[2A Visit 2]] handed in urine samples that they collected themselves in a urine container.\\
+Participants were instructed to collect their urine on the day before their blood was due to be drawn as this provides the possibility to correlate the results between the [[blood_analyses|blood-]] and [[urine_analyses|urine diagnostic tests]]. To facilitate urine collection each participant is provided with a [[https://www.bd.com/en-us/products-and-solutions/products/product-families/bd-vacutainer-complete-urine-collection-kits#productsaccessories|BD Vacutainer®]] 3-liter urine collection container (or equivalent).
-A subset of urine samples were immediately used for standard [[http://en.wikipedia.org/wiki/Clinical_urine_tests|urinalysis]] to assess [[urine analyses|concentrations of proteins]].\\
-The remaining samples were placed into long term [[urine samples storage|storage]] for future analyses.
-===== Sample types =====
+===== Current Study Protocol =====
-Depending on the participant, one of the following urine samples were collected:
+Please note that the text below describes the procedures as they are employed during the ongoing [[3A|third]] assessment. The [[1A|baseline]]- and [[2A|second]] assessments differ in various ways, and Lifelines recommends consulting their respective wiki pages to learn more about the procedures employed at those times.
-  * **24-hour urine samples** are considered the gold standard for defining conditions like albuminuria (Gansevoort RT et al. 2006) and has been the standard of care for assessing kidney function, providing a wide range of reference values. 24-h urine samples were collected by adult (18+) participants in a special container over a full 24-hour period.
-  * An **early morning spot (EMS) urine sample** was handed in by the first 60.000 adult participants of assessment [[1A]], but this practice was discontinued due to inconvenience. Participants emptied their bladder before lying down to sleep, did not urinate during the night, and collected one to two ounces of their first urine from the first urination in the morning in a clean container. First morning urine is the most concentrated and the most likely to detect abnormalities of all urine samples.
-  * A **timed overnight (TO) urine sample** was collected from underage participants (8-17y). Urine was collected during a 12-hour period, starting from 8pm the night before until 8am of the morning of the Lifelines visit.\\
-Participants were instructed to keep the urine sample cool and to return it to Lifelines within 24 hours after collection is completed.\\
+===== Sample Collection =====
-{{:20181205_104431.jpg?200|}}
-{{:20131219_141649.jpg?200|}}
+Adult participants were instructed to collect their urine over a full 24-hour period (22:00-22:00) as these samples are considered the "gold standard" to determine conditions such as albuminuria (Gansevoort RT et al. 2006(([[https://pubmed.ncbi.nlm.nih.gov/16936069/|Evaluation of measures of urinary albumin excretion]]))) and assessing kidney function.\\
+Underage participants (ages 8–17) were instructed to collect their urine over a 12-hour period (20:00-08:00) because of the inconvenience posed by the 3-liter containers.\\
+Participants were instructed to store their containers at room temperature during the sample collection procedure.
-===== Aliquotting =====
+===== Sample Transport =====
-All urine samples were aliquotted in 900ųl fractions using the [[Synchron Urine Aliquoting Robot]].
-Urine samples were processed using two different methods
-  - Plain
-  - Addition of small amounts (~50ų) of EDTA to acidified solutions of ascorbic acid in deionized water, in order to protect vitamins against copper-catalysed aerobic oxidation.
+Participants are instructed to take the urine container with them to their [[3a_visit_2|second appointment]] when their blood is due to be drawn. Lifelines places the containers in a non-chilled transport box. The temperatures in these boxes are continuously monitored ([[logtag|LogTag TRIX-8]]) as part of our [[sample_quality|quality control]] procedures. These transport boxes are then shipped to the Lifelines laboratory, typically arriving between the hours of 10:00–12:00.
-{{:20131219_141743.jpg?200 |}}
+===== Sample Reception =====
-{{:hpim2039.jpg?200 |}}
+Upon arrival at the Lifelines laboratory the urine containers are weighed (which is registered in our laboratory information management system) and subsequently used to fill two sample tubes for further processing:
+  * BD Vacutainer® 6.0 mL Z (No Additive) Plus Urine Tubes, these are sent onwards to the UMCG in a chilled transport box after adding their numbers together.
+  * BD Vacutainer® 11.0 mL Z (No Additive) Plus Urine Tubes
+===== Sample Processing =====
+Urine samples are divided into 900 μl fractions by the [[https://synchronlab.com/product/xperimate-modular-robotic-pipetting-platform/|Synchron Xperimate robot]], a modular robotic pipetting platform. These aliquots can be identified by a unique 2D barcode printed on the bottom of the tubes, and are held within a 1D barcoded rack ([[https://www.lvl-technologies.com/en/sample-storage/safe-2d-tubes-engl/safe-96-it-2D|LVL technologies Safe® 96 IT]] or equivalent). The exact location of each individual sample is logged and continuously tracked in our LIMS.\\
+===== Sample Storage =====
+Aliquots are kept refrigerated (at 4 degrees celsius) until they can be moved into a [[sample_storage|cryofreezer]] (-80 degrees Celsius), which is typically within 10 hours after sample collection.\\

Lifelines Wiki

User Tools

Site Tools

Differences

Page Tools