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--- cryopreserved_white_blood_cells [2019/09/04 11:22] – trynke
+++ cryopreserved_white_blood_cells [2023/01/27 13:01] (current) – outdated information, please refer to cryopreserved_whole_blood instead. kevin
@@ Line 1: / Line 1: @@
-====== Cryopreserved white blood cells ======
-These peripheral blood mononuclear [[https://en.wikipedia.org/wiki/Peripheral_blood_mononuclear_cell|cells]] (PMBC's) are leukocytes isolated from whole blood and comprise of monocytes (macrophage precursors), lymphocytes (both B and T cells), and a small percentage of other immune cells, such as dendritic cells.\\
-===== Protocol =====
-Lifelines isolated PMBC's from freshly drawn [[blood samples|blood]] from xxx participants during [[1A Visit 2]].\\
-Freshly isolated PBMCs were resuspended to 5-10 x 10^6 cells/mL in freezing medium containing 10-20% dimethyl sulfoxide (DMSO) and 40% fetal bovine serum, and were cryopreserved at ultra-low temperatures in liquid nitrogen to remain viable for decades with no significant changes to viability or functionality.\\
-Note that DMSO, used as a cryoprotectant to reduce the formation of ice crystals and prevent cell damage, is toxic to cells at higher temperature and must be washed off immediately once cells are thawed.\\
-After a careful thawing procedure, cryopreserved (c-) PBMCs can be stimulated or treated with various immunomodulatory agents or drugs in vitro.
-{{:hpim2036.jpg?200|}}
-{{:136091-004-c22a66a2.jpg?200|}}
-{{:cryotube.png?200|}}