blood_samples
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blood_samples [2020/05/18 14:09] trynke |
blood_samples [2023/01/27 14:00] kevin |
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| - | ====== Blood plasma samples ====== | + | ====== Blood samples ====== |
| Fasting blood was collected from all participants aged 8 years or older that participated in [[1A Visit 2]] and [[2A Visit 2]] (and currently in [[3A visit 2]]).\\ | Fasting blood was collected from all participants aged 8 years or older that participated in [[1A Visit 2]] and [[2A Visit 2]] (and currently in [[3A visit 2]]).\\ | ||
| Blood was collected in various blood tubes (see below), transported from the Lifelines [[location|locations]] to the central lab in Groningen, centrifuged, aliquotted, and stored at -80°C on the day of collection.\\ | Blood was collected in various blood tubes (see below), transported from the Lifelines [[location|locations]] to the central lab in Groningen, centrifuged, aliquotted, and stored at -80°C on the day of collection.\\ | ||
| - | A subset of blood samples was used for standard [[blood analyses]]. The remaining samples were placed in long term [[blood samples storage|storage]] to be used by scientists for future analyses.\\ | + | A subset of plasma samples was used for standard [[blood analyses]]. The remaining samples were placed in long term [[blood samples storage|storage]] to be used by scientists for future analyses.\\ |
| Note that [[urine samples]] were collected on the same day. | Note that [[urine samples]] were collected on the same day. | ||
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| * **EDTA samples** are collected in vacutainers with [[https://en.wikipedia.org/wiki/Ethylenediaminetetraacetic_acid|EDTA]] anticoagulants used to prevent clot formation both in vitro and in vivo, in order to maintain blood in the fluid state for hematological testing or to obtain suitable plasma for coagulation and clinical chemistry analyses. | * **EDTA samples** are collected in vacutainers with [[https://en.wikipedia.org/wiki/Ethylenediaminetetraacetic_acid|EDTA]] anticoagulants used to prevent clot formation both in vitro and in vivo, in order to maintain blood in the fluid state for hematological testing or to obtain suitable plasma for coagulation and clinical chemistry analyses. | ||
| * **Heparin samples** are collected in vacutainers with [[https://en.wikipedia.org/wiki/Heparin|sodium heparin]] as an anticoagulant in order to prepare heparinized whole blood. | * **Heparin samples** are collected in vacutainers with [[https://en.wikipedia.org/wiki/Heparin|sodium heparin]] as an anticoagulant in order to prepare heparinized whole blood. | ||
| - | * **Citrate samples** are collected in vacutainers with citric acid, a reversible anticoagulant to enable coagulation assays. | + | * **Citrate samples** are collected in vacutainers with citric acid, a reversible anticoagulant to enable coagulation assays (only available from [[1A visit 2|1A]]). |
| * **[[https://en.wikipedia.org/wiki/Buffy_coat|Buffy coat]] samples** are collected from the fraction of an anticoagulated blood sample that contains most of the white blood cells and platelets, using density gradient centrifugation. | * **[[https://en.wikipedia.org/wiki/Buffy_coat|Buffy coat]] samples** are collected from the fraction of an anticoagulated blood sample that contains most of the white blood cells and platelets, using density gradient centrifugation. | ||
| - | * **[[Cryopreserved white blood cells|PMBC's]]** - peripheral blood mononuclear leukocytes isolated from whole blood. | ||
| - | |||
| - | All blood sample types except for PCMB's are available for virtually all participants that participated in [[1A visit 2]] and [[2A visit 2]]. | ||
| {{:20181116_140050.jpg?200|}} | {{:20181116_140050.jpg?200|}} | ||
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| ===== Aliquotting ===== | ===== Aliquotting ===== | ||
| - | All blood samples are aliquotted into 900ųl fractions prior to storage.\\ | + | All blood samples are aliquotted into 900ųl fractions prior to [[Blood samples storage|storage]].\\ |
| Each aliquot is identified by a unique [[https://en.wikipedia.org/wiki/Data_Matrix| 2D code]].\\ | Each aliquot is identified by a unique [[https://en.wikipedia.org/wiki/Data_Matrix| 2D code]].\\ | ||
| {{:20181123_135707.jpg?200|}}\\ | {{:20181123_135707.jpg?200|}}\\ | ||
blood_samples.txt · Last modified: 2023/03/23 14:19 by kevin
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