All Lifelines participants of 8 years and older participating in general assessment 1A (“Baseline”) visited a location of their choice.

• Total duration: 70 minutes
• Research assistants (= nurse practitioners) received the participants
• Informed Consent and basic personal information was checked
• 1A Questionnaire 1 was taken in and checked (also during blood pressure measurement)
• If participant is 65+ years old: MMSE was performed
• All instruments were checked if ready for use
• The following measurements were done in order according to their respective SOP’s:
  1. Anthropometry
  2. Blood pressure
  3. ECG
  4. Pulmonary function test or Ruff Figural Fluency Test
  5. MINI neuropsychiatric interview
  6. Skin autofluorescence
• Possible changes in the order:
  • Blood pressure and ECG could be swapped
  • Pulmonary function test could be done after the MINI
• For the most part of 1A, the Pulmonary function test and Ruff Figural Fluency Test were performed in alternate weeks so that participants underwent only one of these two tests.
• In case of temporary counter indications of the participant or technical/logistic problems, the tests could be rescheduled for 1A Visit 2

• Total duration: 60 min (plus 30-45 minutes for on-site 1A Youth Questionnaire)
• Research assistants (= nurse practitioners) received the participants in a child-friendly manner
• Informed Consent must have been checked prior to the visit
• 1A Questionnaire 1 was taken in and checked (also during blood pressure measurement)
• If participant is 65+ years old: MMSE was performed
• All instruments were checked if ready for use
• The following measurements were done in order according to their respective SOP’s:
  1. Anthropometry
  2. Blood pressure
  3. ECG
  4. Pulmonary function test or Ruff Figural Fluency Test
  5. MINI neuropsychiatric interview
  6. Skin autofluorescence
• Possible changes in the order:
  • Blood pressure and ECG could be swapped
  • Pulmonary function test could be done after the MINI
• For the most part of 1A, the Pulmonary function test and Ruff Figural Fluency Test were performed in alternate weeks so that participants underwent only one of these two tests.
• In case of temporary counter indications of the participant or technical/logistic problems, the tests could be rescheduled for 1A Visit 2

1A Visit 1 was accompanied by 1A Questionnaire 1 which was filled in, on average, within xx days (+/- yy days) prior to visit 1.
1A Visit 1 was followed by 1A Visit 2 within, on average, 40 days.

1A Visit 1 is procedurally equivalent to 2A Visit 1 and 3A Visit 1. The average time between 1A Visit 1 and 2A Visit 1 was 3 years and 11 months.